PRE-CONGRESS PROGRAM
Pre-congress workshop on Flow cytometry (07– 09 December 2024)
Faculté des Sciences de la Santé (FSS), Cotonou, Benin
Organizers: Benin and Gambian Immunology Societies in collaboration with Becton Dickinson Biosciences
Trainers and Speakers:
- Fabien Prodjinotho (Technical University of Munich, Germany)
- Martin Goodier (Medical Research Council Unit, The Gambia & LSHTM-UK)
- Ya-Jankey Jagne (Medical Research Council Unit, The Gambia & LSHTM-UK)
- Serena Di Cecilia (Becton Dickinson Biosciences)
- Holden Maecker (Stanford University, USA)
- Claude Lambert (CHU St Etienne, IFCC Working Group, France)
- Jainaba Njie-Jobe (Medical Research Council Unit, The Gambia & LSHTM-UK)
| Time | ||
|---|---|---|
| SATURDAY 07 DECEMBER Introduction to Flow cytometry, the cytometer and applications |
||
| 08:30 – 09:00 | Introduction to the course and official opening | T. Adjobimey / F. Prodjinotho / FAIS official |
| 09:00 – 09:30 | Short introduction round by participants | F. Prodjinotho |
| 09:30 – 11:00 | Introduction to Flow cytometry and applications | C. Lambert |
| 11:00 – 11:30 | Coffee break | |
| 11:30 – 12:30 | The Cytometer: Fluidics, optics and electronics | H. Maecker |
| 12:30 – 14:00 | Lunch | |
| 14:00 – 15:30 | The Reagents: Antibodies and fluorescent dyes | M. Goodier / C. Lambert |
| 15:30 – 16:00 | Coffee break | |
| 16:00 – 17:30 | Trouble shooting session: Tips and tools for flow cytometry sample preparation success | F. Prodjinotho / Y. Jagne / M. Goodier/J. Njie-Jobe |
| SUNDAY 08 DECEMBER Sample types, panels, good staining practice and instrument maintenance |
||
| 08:30 – 09:30 | The Panel: Understanding the basics of spillover and compensation | C. Lambert |
| 09:30 – 11:00 | The panel: Panel design tools | M. Goodier |
| 11:00 – 11:30 | Coffee break | |
| 11:30 – 12:30 | The Samples: Best practices for classical staining protocols | F. Prodjinotho |
| 12:30 – 14:00 | Lunch | |
| 14:00 – 15:00 | The Samples: Guidelines for controls and their uses | Y. Jagne/J. Njie-Jobe |
| 15:00 – 16:00 | Quick introduction to basic and high dim analyses | C. Lambert /F. Prodjinotho |
| 16:00 – 16:30 | Coffee break | |
| 16:30 – 17:30 | Troubleshooting session 2: Best practices for instrument maintenance and troubleshooting | H. Maecker / M. Goodier / F. Prodjinotho/ Y. Jagne/ J. Njie-Jobe |
| MONDAY 09 DECEMBER Data processing, quality control and advanced analysis |
||
| 09:00 – 11:00 | The Data and analysis: FlowJo basic tools (workspace, data QC, compensation, gating etc) | S. Di Cecilia |
| 11:00 – 11:30 | Coffee break | |
| 11:30 – 13:30 | Data and analysis: FlowJo advanced tools for high dimensional analysis (installation, QC plugins, reduce dimensionality) | S. Di Cecilia |
| 13:30 – 14:30 | Lunch | |
| 14:30 – 16:00 | Visit of IRD Research Center and Flow cytometry platform and cytometer demo | |
| 16:00 – 16:30 | Coffee break | |
| 16:30 – 17:30 | Practical session for FlowJo basic and advanced tools (gating strategies, analysis, QC & reduce dimensionality) | M. Goodier / C. Lambert /H. Maecker / Y. Jagne / F. Prodjinotho/ Njie-Jobe |
| 17:30 – 18:00 | Round discussion, feedback and closing remarks | |
